Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
Rabbit IgG (H&L) antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Donkey anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Donkey anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation
Reactivity
Rabbit
Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with Rabbit IgG whole molecule in donkey
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1 mg/mL
Dilution
ELISA: 1:10,000 - 1:20,000, IHC: 1:400 - 1:2,000, WB: 1:1,000 - 1:5,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency. 0 moles Alkaline Phosphatase (AP) per mole
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Donkey
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog