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Rabbit IgG Fc Antibody Peroxidase Conjugated

Rabbit IgG F (c) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Donkey anti-Rabbit IgG F (c) Antibody peroxidase Conjugation, Donkey anti-Rabbit IgG F (c) HRP Conjugated Antibody

Reactivity

Rabbit

Immunogen

Rabbit IgG F (c) fragment

Clonality

Polyclonal

Conjugation

HRP

Concentration

2.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG, Rabbit IgG F (c) and Rabbit Serum. No reaction was observed against Rabbit IgG F (ab) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Rabbit IgG F (c) Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Donkey

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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