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Rabbit IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Rabbit IgG (H&L) antibody (TRITC)

Product Specifications

Product Name Alternative

Donkey anti-Rabbit IgG Antibody Rhodamine Conjugation, Donkey anti-Rabbit IgG Rhodamine Conjugated Antibody

Reactivity

Rabbit

Immunogen

Rabbit IgG whole molecule

Clonality

Polyclonal

Conjugation

TRITC

Concentration

1.0 mg/mL

Dilution

FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Form

Lyophilized

References & Citations

Nishimura, Kei et al. Glucagon-like peptide-1 is co-localized with neurotensin in the chicken ileum Cell Tissue Res, 368, 277-286 (2017)

Pubmed id

28108848

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Rabbit IgG (H&L) Rhodamine Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

FC, FLISA, IF

Host or Source

Donkey

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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