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Rabbit IgG (H&L) Antibody

Rabbit IgG (H&L) antibody

Product Specifications

Product Name Alternative

Sheep Anti-Rabbit IgG, Rabbit Secondary Ab, anti-Rabbit IgG Antibody in Sheep

Reactivity

Rabbit

Immunogen

Rabbit IgG whole molecule

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

2.1 mg/mL

Dilution

ELISA: 1:40,000 - 1:200,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000

Purity

Anti-RABBIT IgG (H&L) (SHEEP) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Rabbit IgG and Rabbit Serum.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-RABBIT IgG (H&L) (SHEEP) Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Sheep

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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