Rabbit IgG (H&L) Antibody Pre-adsorbed
RABBIT IgG (H&L) antibody
Product Specifications
Product Name Alternative
Mouse anti-Rabbit IgG Antibody, mouse anti-Rabbit IgG pre-adsorbed Antibody
Reactivity
Rabbit
Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in mouse.
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.1 mg/mL
Dilution
ELISA: 1:65,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:30,000
Purity
This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human or Mouse Serum Proteins.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Rabbit IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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