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Rabbit IgG (H&L) Antibody Pre-adsorbed

RABBIT IgG (H&L) antibody

Product Specifications

Product Name Alternative

Mouse anti-Rabbit IgG Antibody, mouse anti-Rabbit IgG pre-adsorbed Antibody

Reactivity

Rabbit

Immunogen

Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in mouse.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.1 mg/mL

Dilution

ELISA: 1:65,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:30,000

Purity

This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human or Mouse Serum Proteins.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Rabbit IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

Host or Source

Mouse

Preservative

Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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