Rabbit IgG F (ab') 2 Antibody Alkaline Phosphatase Conjugated
Rabbit IgG F (ab') 2 antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Goat Anti-Rabbit IgG F (ab') 2 Antibody alkaline phosphatase Conjugation, Goat Anti-Rabbit IgG Fab2 alkaline phosphatase Conjugated Antibody, Goat Anti-Rabbit IgG Fab2 Fragment alk phos Conjugated Antibody
Reactivity
Rabbit
Immunogen
Rabbit IgG F (ab') 2 fragment
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
0.75 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG, Rabbit IgG F (ab’) 2 and Rabbit Serum. No reaction was observed against Rabbit IgG F (c) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG
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