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Rabbit IgG F (ab') 2 Antibody Peroxidase Conjugated

Rabbit IgG F (ab') 2 antibody (Peroxidase)

Product Specifications

Product Name Alternative

Goat Anti-Rabbit IgG F (ab') 2 Antibody peroxidase Conjugation, Goat Anti-Rabbit IgG Fab2 peroxidase Conjugated Antibody, Goat Anti-Rabbit IgG Fab2 Fragment HRP Conjugated Antibody

Reactivity

Rabbit

Immunogen

Rabbit IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

HRP

Concentration

2.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F (ab’) 2 and Rabbit Serum. No reaction was observed against Rabbit IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Rabbit IgG F (ab') 2 peroxidase conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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