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Rabbit IgG (H&L) Antibody

Rabbit IgG (H&L) antibody

Product Specifications

Product Name Alternative

Goat anti-Rabbit IgG Antibody, goat anti Rabbit IgG

Reactivity

Rabbit

Immunogen

Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

2.20 mg/mL

Dilution

ELISA: 1:10,000 - 1:60,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000

Purity

Rabbit Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Form

Liquid (sterile filtered)

Storage Conditions

Store Rabbit Secondary Antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Rabbit IgG (H&L) has been tested by ELISA, dot blot, and western blot. This antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user

Tested Applications

DOT, ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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