Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
Mouse IgG (H&L) antibody (Biotin)
Product Specifications
Product Name Alternative
Rabbit anti-Mouse IgG Antibody biotin conjugation, rabbit anti-Mouse IgG biotin conjugated Antibody
Reactivity
Mouse
Immunogen
Mouse IgG whole molecule
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:300,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
Mouse secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Form
Lyophilized
Storage Conditions
Store secondary antibody conjugate at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Mouse secondary antibody conjugated to biotin is available in a variety of formats. Anti-Mouse IgG Biotin Antibody has been tested by ELISA and is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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