Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated
Selected Variant
GTR18185606
Size: 2 mg
Mouse IgG (H&L) antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Rabbit Anti-Mouse IgG Secondary Antibody HRP Conjugated, RAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
Reactivity
Mouse
Immunogen
Mouse IgG whole molecule
Clonality
Polyclonal
Conjugation
HRP
Concentration
2.0 mg/mL
Dilution
ELISA: 1:50,000 - 1:300,000, IHC: 1:500 - 1:2,500, WB: 1:10,000 - 1:30,000
Purity
HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Form
Lyophilized
Storage Conditions
Store mouse secondary antibody conjugate at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in rabbit detects specifically Mouse IgG whole molecule. Anti-Mouse IgG peroxidase antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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