Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated
Mouse IgG (H&L) antibody (FITC)
Product Specifications
Product Name Alternative
Rabbit Anti-Mouse IgG Antibody fluorescein Conjugation, Rabbit Anti-Mouse IgG Antibody fluorescein Conjugated, Rabbit Anti-Mouse IgG FITC Conjugated Antibody
Reactivity
Mouse
Immunogen
Mouse IgG whole molecule
Clonality
Polyclonal
Conjugation
FITC
Concentration
2.0 mg/mL
Dilution
FLISA: 1:10,000 - 1:50,000, IF: 1:1,000 - 1:5,000
Purity
Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Form
Lyophilized
Storage Conditions
Store Conjugated Second Antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Mouse IgG Fluorescein Antibody has been tested by dot blot and western blot and is designed for flow cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Tested Applications
DOT, FLISA, IF, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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