Mouse IgG3 (Gamma 3 chain) Antibody
Mouse IgG3 (Gamma 3 chain) antibody
Product Specifications
Product Name Alternative
Rabbit Anti-Mouse IgG3 (Gamma 3 chain) Antibody, Rabbit Anti Mouse IgG3 Antibody
Reactivity
Mouse
Immunogen
Mouse IgG3 heavy chain
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Concentration
1.2 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:5,000
Purity
Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG3 coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG3. Specificity was confirmed by ELISA. For IgG1, IgG2a and IgG2b reactivity was less than 5% against target (IgG3, IgG) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-Mouse IgG3 antibody has been tested by ELISA and dot blot and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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