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Mouse IgG2a Antibody

MOUSE IgG2a antibody

Product Specifications

Product Name Alternative

Rabbit Anti-Mouse IgG2a Antibody, Mouse IgG2a Secondary Antibody, Rb-a-mouse IgG2a

Reactivity

Mouse

Immunogen

Mouse IgG2a heavy chain

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.02 mg/mL

Dilution

ELISA: 1:15,000 - 1:30,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000

Purity

Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG2a. Specificity was confirmed by ELISA. For IgG1, IgG2b and IgG3 reactivity was less than 5% against target (IgG2a, IgG) .

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Tested Applications

DOT, ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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