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Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody

Mouse IgG (gamma 1, 2a, 2b and 3 chain) antibody

Product Specifications

Product Name Alternative

Rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody, rabbit anti-Mouse IgG Antibody

Reactivity

Mouse

Immunogen

Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

1.0 mg/ml

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Mouse IgG subclass pan reactive Secondary Antibody has been tested by ELISA and is designed for ELISA, Immunohistochemistry, western blotting as well as other anti-IgG antibody based assays. Mouse IgG secondary antibody is available in a variety of formats.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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