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Mouse IgG (H&L) Secondary Antibody Biotin Conjugated Pre-Adsorbed

Mouse IgG (H&L) antibody (Biotin)

Product Specifications

Product Name Alternative

Goat Anti-Mouse IgG Secondary Antibody biotin Conjugation, Goat Anti-Mouse IgG Antibody biotin Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG biotin conjugated secondary antibody

Reactivity

Mouse

Immunogen

Mouse IgG whole molecule

Clonality

Polyclonal

Conjugation

Biotin

Concentration

2.0 mg/mL

Dilution

ELISA: 1:550,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

Anti Mouse IgG antibody conjugated to biotin was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. Specificity was confirmed by ELISA at less than 1.0% cross reactivity against human IgG.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Mouse secondary antibody conjugated to Biotin is available in a variety of formats. Anti-Mouse IgG Biotin Antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti mouse antibody based assays.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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