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Mouse IgG Fc Antibody Alkaline Phosphatase Conjugated

Mouse IgG F (c) antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Goat Anti-Mouse IgG F (c) alkaline phosphatase Conjugated Antibody, Goat Anti-Mouse IgG Fc Antibody alk phos Conjugation, Goat Anti-Mouse IgG Fc Fragment Antibody ALP Conjugated

Reactivity

Mouse

Immunogen

Mouse IgG F (c) fragment

Clonality

Polyclonal

Conjugation

AP

Field of Research

Signal Transduction

Concentration

0.81 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG, Mouse IgG F (c) and Mouse Serum. No reaction was observed against Mouse IgG F (ab) .

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Anti-Mouse IgG F (c) Alkaline Phosphatase has been tested by ELISA and assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:5,000 of the reconstitution concentration is suggested for this product.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG

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