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Mouse IgG IgA IgM (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Mouse IgG IgA IgM (H&L) antibody (Biotin)

Product Specifications

Product Name Alternative

Goat Anti Mouse IgG IgA IgM Antibody Biotin Conjugation, Goat Anti-Mouse IgG IgA IgM Biotin conjugated Antibody, Goat Anti-Mouse IgGAM Biotin conjugate

Reactivity

Mouse

Immunogen

Mouse IgG IgA and IgM whole molecule

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:300,000, IHC: 1:1,500 - 1:15,000, WB: 1:3,000 - 1:30,000

Purity

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all mouse isotypes and chain combinations. ELISA was used to confirm specificity at less than 1% of the target signal.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Mouse IgG IgA IgM Biotin Antibody has been tested by ELISA and is useful for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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