Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
Mouse IgG (H&L) antibody (FITC)
Product Specifications
Product Name Alternative
Goat Anti-Mouse IgG Secondary Antibody fluorescein Conjugated, Goat Anti-Mouse IgG Antibody FITC Conjugated, GAM-FITC, Anti-mouse IgG secondary antibody, anti-mouse IgG Fluorescein conjugated secondary antibody
Reactivity
Mouse
Immunogen
Mouse IgG whole molecule
Clonality
Polyclonal
Conjugation
FITC
Concentration
1.0 mg/mL
Dilution
FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Purity
Goat Anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
Form
Lyophilized
Storage Conditions
Store anti mouse secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Mouse IgG Fluorescein Antibody has been tested by dot blot and western blot and is ideal for Fluorescent Western Blot, FLISA, Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy as well as other antibody detection methods.
Tested Applications
DOT, FC, FLISA, IF, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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