Mouse IgG2b Antibody Pre-adsorbed

Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2b coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG1, IgG2a and IgG3 reactivity was less than 5% against target (IgG2b, IgG) . Cross-reactivity against Bovine is assessed only by IEP.

Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Mouse IgG2b secondary antibody has been tested by dot blot and ELISA and is available in a variety of formats. Anti-Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays.