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Human IgG (H&L) Antibody

Human IgG (H&L) antibody

Product Specifications

Product Name Alternative

Sheep Anti Human IgG Antibody, Sheep Anti-Human IgG Antibody

Reactivity

Human

Immunogen

Human IgG whole molecule

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

2.1 mg/mL

Dilution

ELISA: 1:30,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:20,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Human IgG and Human Serum.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Human IgG antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Sheep

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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