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Human IgG Fc Antibody Biotin Conjugated

Human IgG F (c) antibody (Biotin)

Product Specifications

Product Name Alternative

Human IgG F (c) Antibody, Rb-a-Human Biotin conjugated, Human IgG F (c) Antibody in rabbit, Rabbit anti-human IgG F (c) Biotin conjugated Secondary Antibody

Reactivity

Human

Immunogen

Anti-Human IgG F (c) was produced by repeated immunization with human IgG F (c) fragment in rabbit.

Clonality

Polyclonal

Conjugation

Biotin

Concentration

2.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgG, Human IgG F (c) and Human Serum. No reaction was observed against Human IgG F (ab) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Human IgG F (c) Biotin conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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