Human IgG F (ab') 2 Antibody Alkaline Phosphatase Conjugated
Human IgG F (ab') 2 antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Rabbit Anti-Human IgG Fab2 Antibody alkaline phosphatase Conjugated, Rabbit Anti-Human IgG F (ab') 2 Antibody alkaline phosphatase Conjugated, Rabbit Anti-Human IgG Fab2 Fragment Antibody alk phos Conjugated
Reactivity
Human
Immunogen
Human IgG F (ab') 2 fragment
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1.0 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F (ab’) 2 and Human Serum. No reaction was observed against Human IgG F (c) .
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 is suggested for this product.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG
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