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Human IgG Fc Antibody Alkaline Phosphatase Conjugated

Human IgG F (c) antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Human Fc Antibody, Rb-a-Human F (c), Human IgG F (c) Antibody in Rabbit, rabbit anti-Human IgG F (c) Alkaline phosphatase Secondary Antibody

Reactivity

Human

Immunogen

Anti-Human IgG F (c) was produced by repeated immunization with Human Fc fragment in rabbit.

Clonality

Polyclonal

Conjugation

AP

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500

Purity

Anti-Human IgG F (c) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F (c) and Human Serum. No reaction was observed against Human IgG F (ab) .

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Anti-Human IgG F (c) Alkaline Phosphatase Conjugated antibody has been tested by dot blot and ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Tested Applications

DOT, ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG

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