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Human IgG F (ab') 2 Antibody Peroxidase Conjugated

Human IgG F (ab') 2 antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit Anti-Human IgG Fab2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG F (ab') 2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG Fab2 Fragment Antibody HRP Conjugated

Reactivity

Human

Immunogen

Human IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

HRP

Concentration

2.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000

Purity

Anti-Human IgG F (ab') 2 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F (ab’) 2 and Human Serum. No reaction was observed against Human IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Human IgG F (ab') 2 Antibody has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for Anti-Human IgG F (ab') 2 Antibody.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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