Human IgG F (ab') 2 Antibody Peroxidase Conjugated
Human IgG F (ab') 2 antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit Anti-Human IgG Fab2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG F (ab') 2 Antibody peroxidase Conjugated, Rabbit Anti-Human IgG Fab2 Fragment Antibody HRP Conjugated
Reactivity
Human
Immunogen
Human IgG F (ab') 2 fragment
Clonality
Polyclonal
Conjugation
HRP
Concentration
2.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Purity
Anti-Human IgG F (ab') 2 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F (ab’) 2 and Human Serum. No reaction was observed against Human IgG F (c) .
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Human IgG F (ab') 2 Antibody has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for Anti-Human IgG F (ab') 2 Antibody.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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