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Human IgG (gamma chain) Antibody Fluorescein Conjugated

Human IgG (gamma chain) antibody (FITC)

Product Specifications

Product Name Alternative

Rabbit anti-Human IgG (gamma chain) fluorescein conjugated Antibody, rabbit anti-Human IgG Antibody FITC conjugation

Reactivity

Human

Immunogen

Human IgG gamma heavy chain

Clonality

Polyclonal

Conjugation

FITC

Concentration

1.0 mg/mL

Dilution

FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Human IgA or Human IgM. Specificity was confirmed by ELISA minimal cross reactivity against other Human heavy or light chain isotypes.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Human IgG (gamma chain) Fluorescein has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

DOT, FC, FLISA, IF, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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