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Human IgG Fc Antibody

Human IgG F (c) antibody

Product Specifications

Product Name Alternative

Human IgG F (c) Antibody, Human IgG Fc Antibody, Rb-a-Human IgG Fc, Human IgG F (c) Antibody in Rabbit, rabbit anti-human IgG F (c) Secondary Antibody

Reactivity

Human

Immunogen

Anti-Human IgG F (c) fragment was produced by repeated immunization with Human IgG F (c) fragment in rabbit.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

5.014 mg/mL

Dilution

ELISA: 1:26,000-1:46,000, IHC: 1:1,000 - 1:6,000, WB: 1:3,000 - 1:30,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F (c) and Human Serum. No reaction was observed against Human IgG F (ab) .

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Human IgG F (c) fragment has been tested by western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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