Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Human IgG (H&L) antibody (Alkaline Phosphatase)
Product Specifications
Product Name Alternative
Mouse anti-Human IgG alkaline phosphatase conjugated Antibody, mouse anti-Human IgG Antibody alk phos conjugation
Reactivity
Human
Immunogen
Human IgG whole molecule
Clonality
Polyclonal
Conjugation
AP
Field of Research
Signal Transduction
Concentration
1 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
Purity
This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Alkaline Phosphatase, Human IgG and Human Serum.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Notes
For research use only.
Applications Notes
Anti-Human IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Mouse
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Isotype
IgG
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