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Horse IgG F (ab') 2 Antibody Alkaline Phosphatase Conjugated

Selected Variant

GTR18185361

Size: 1 mg

Horse IgG F (ab') 2 antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Rabbit Anti-Horse IgG Fab2 Fragment alkaline phosphatase Conjugated Antibody, Rabbit Anti-Horse IgG F (ab') 2 Antibody alkaline phosphatase conjugate, Rabbit Anti-Horse IgG Fab2 Antibody alk phos Conjugation

Reactivity

Equine

Immunogen

Horse IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

AP

Field of Research

Signal Transduction

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:8,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F (ab') 2 and Horse Serum. No reaction was observed against Horse IgG F (c) .

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Anti-Horse IgG F (ab') 2 Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG

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