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Golden Syrian Hamster IgG F (ab') 2 Antibody Biotin Conjugated

Selected Variant

GTR18185318

Size: 1 mg

Golden Syrian Hamster IgG F (ab') 2 antibody (Biotin)

Product Specifications

Product Name Alternative

Rabbit anti-Golden Syrian Hamster IgG F (ab') 2 fragment biotin conjugated Antibody, rabbit anti-Golden Syrian Hamster IgG Fab2 fragment biotin conjugated Antibody, rabbit anti-Hamster IgG F (ab') 2 biotin conjugated Antibody

Reactivity

Golden Hamster

Immunogen

Hamster IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.0 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Hamster IgG, Hamster IgG F (ab’) 2 and Hamster Serum. No reaction was observed against Hamster IgG F (c) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This product has been assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 1.0 mL

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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