Guinea Pig IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
Selected Variant
GTR18185286
Size: 1 mg
Guinea Pig IgG (H&L) antibody (Peroxidase)
Product Specifications
Product Name Alternative
Donkey Anti-Guinea Pig IgG Peroxidase Conjugation, Donkey Anti-Guinea Pig IgG HRP Conjugated
Reactivity
Guinea pig
Immunogen
Guinea Pig IgG whole molecule
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Neuroscience
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Guinea Pig IgG and with light chains of most Guinea Pig immunoglobulins.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Guinea Pig IgG Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
ELISA, WB
Host or Source
Donkey
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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