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Guinea Pig IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Selected Variant

GTR18185286

Size: 1 mg

Guinea Pig IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Donkey Anti-Guinea Pig IgG Peroxidase Conjugation, Donkey Anti-Guinea Pig IgG HRP Conjugated

Reactivity

Guinea pig

Immunogen

Guinea Pig IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Field of Research

Neuroscience

Concentration

1.0 mg/mL

Dilution

ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Guinea Pig IgG and with light chains of most Guinea Pig immunoglobulins.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Guinea Pig IgG Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

ELISA, WB

Host or Source

Donkey

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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