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Goat IgG (H&L) Antibody Pre-Adsorbed

Goat IgG (H&L) antibody

Product Specifications

Product Name Alternative

Donkey anti-goat IgG antibody pre-adsorbed

Reactivity

Goat

Immunogen

Goat IgG whole molecule

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Concentration

1.07 mg/mL

Dilution

ELISA: 1:150,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat Serum Proteins. Specificity was confirmed using ELISA minimal cross reactivity against the targets stated above.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Goat IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Tested Applications

ELISA, IHC, WB

Host or Source

Donkey

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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