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Goat IgG F (ab') 2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Goat IgG F (ab') 2 antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Rabbit anti-goat IgG F (ab') 2 ALP conjugated, rabbit anti-goat IgG F (ab') 2 alkaline phosphatase conjugated, rabbit anti-goat IgG Fab2 ALP conjugated, rabbit anti-goat IgG Fab2 alkaline phosphatase conjugated

Reactivity

Goat

Immunogen

Goat IgG F (ab') 2 fragment

Clonality

Polyclonal

Conjugation

AP

Field of Research

Cell Biology

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG, Goat IgG F (ab') 2 and Goat Serum. No reaction was observed against Goat IgG F (c) or Human Serum Proteins.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

This product has been assayed against 1.0 μg of Goat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 is suggested for this product.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG

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