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GOAT IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated

GOAT IgG (H&L) antibody (Alkaline Phosphatase)

Product Specifications

Product Name Alternative

Rabbit anti-Goat IgG Alkaline Phosphatase Conjugated Antibody, rabbit anti-Goat IgG Antibody alk phos Conjugation

Reactivity

Goat

Immunogen

Goat IgG whole molecule

Clonality

Polyclonal

Conjugation

AP

Field of Research

Cell Biology

Concentration

1.0 mg/mL

Dilution

ELISA: 1:2,000 - 1:13,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500

Purity

Anti-Goat Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG and Goat Serum.

Form

Liquid (sterile filtered)

Storage Conditions

Store secondary antibody at 4° C before opening. DO NOT FREEZE. Anti-Goat secondary antibodies are stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Notes

For research use only.

Applications Notes

Anti-Goat IgG Alkaline Phosphatase conjugate has been tested by ELISA, dot blot, and western blot and is suitable for immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

DOT, ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.09% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Isotype

IgG

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