Goat IgG (H&L) Secondary Antibody Peroxidase Conjugated
GOAT IgG (H&L) antibody (Peroxidase)
Product Specifications
Product Name Alternative
Rabbit anti-Goat IgG Peroxidase Conjugated Antibody, rabbit anti-Goat IgG HRP Conjugated Antibody, HRP anti-goat IgG, Peroxidase anti-Goat IgG
Reactivity
Goat
Immunogen
Goat IgG whole molecule
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Cell Biology
Concentration
2.0 mg/mL
Dilution
ELISA: 1:50,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:20,000
Purity
Rabbit Anti-Goat IgG secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.
Form
Lyophilized
Storage Conditions
Store Secondary Antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Goat IgG Peroxidase conjugate has been tested by ELISA and western blot. This antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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