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Goat IgG (H&L) Secondary Antibody Fluorescein Conjugated

Goat IgG (H&L) antibody (FITC)

Product Specifications

Product Name Alternative

FITC Conjugated Secondary Antibody, Anti-Goat FITC, rabbit anti-goat IgG fluorescein conjugated antibody

Reactivity

Goat

Immunogen

Goat IgG whole molecule

Clonality

Polyclonal

Conjugation

FITC

Field of Research

Cell Biology

Concentration

2.0 mg/mL

Dilution

FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000

Purity

Anti-Goat Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum.

Form

Lyophilized

Storage Conditions

Store anti-goat FITC conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Goat IgG Fluorescein Antibody has been tested by dot blot and western blot. Secondary antibody reagents are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods

Tested Applications

DOT, FC, FLISA, IF, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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