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Chicken IgG (H&L) Secondary Antibody Biotin Conjugated

Chicken IgG (H&L) antibody (Biotin)

Product Specifications

Product Name Alternative

Rabbit anti-Chicken IgG Biotin Conjugated Antibody, Rabbit anti-Chicken IgG Antibody Biotin Conjugation, Chicken Secondary Antibody, Rabbit anti-Chicken IgY Antibody Biotin

Reactivity

Gallus

Immunogen

Anti-Chicken IgY / IgG whole molecule was produced by repeated immunization with Chicken IgY / IgG whole molecule in rabbit.

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.5 mg/mL

Dilution

ELISA: 1:500,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000

Purity

Biotin Conjugated Anti-Chicken IgG / IgY secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG / IgY coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Chicken IgG / IgY. Some light chain cross reactivity may be observed against chicken immunoglobulins.

Form

Lyophilized

Storage Conditions

Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Secondary antibodies are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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