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Chicken IgG (H&L) Antibody Peroxidase Conjugated

Chicken IgG (H&L) antibody (Peroxidase)

Product Specifications

Product Name Alternative

Rabbit anti-Chicken IgG Antibody Peroxidase Conjugation, rabbit anti-Chicken IgG HRP Conjugated Antibody, rabbit anti-Chicken IgY HRP

Reactivity

Gallus

Immunogen

Chicken IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Concentration

1.0 mg/mL

Dilution

ELISA: 1:25,000 - 1:75,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,500 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

Anti-Chicken IgG Peroxidase conjugate has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Tested Applications

DOT, ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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