Chicken IgG (H&L) Secondary Antibody Fluorescein Conjugated
CHICKEN IgG (H&L) antibody (FITC)
Product Specifications
Product Name Alternative
Rabbit anti-Chicken IgG Antibody Fluorescein Conjugation, rabbit anti-Chicken IgG FITC Conjugated Antibody, rabbit anti-Chicken IgY Antibody Fluorescein Conjugation, rabbit anti-Chicken IgY FITC Conjugated Antibody
Reactivity
Gallus
Immunogen
Chicken IgG whole molecule
Clonality
Polyclonal
Conjugation
FITC
Concentration
1.0 mg/mL
Dilution
FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-Chicken IgG Fluorescein Antibody has been tested by dot blot and western blot. This product is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F (c) mediated binding, lot-to-lot consistency, high titer and specificity.
Tested Applications
DOT, FC, FLISA, IF, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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