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Chicken IgG (H&L) Antibody

Chicken IgG (H&L) antibody

Product Specifications

Product Name Alternative

Rabbit anti-Chicken IgG Antibody, Chicken Secondary Antibody, Rabbit anti-Chicken IgY

Reactivity

Gallus

Immunogen

Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in rabbit.

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

10.14 mg/mL

Dilution

ELISA: 1:200,000 - 1:400,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:30,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Notes

For research use only.

Applications Notes

Anti-Chicken IgG (H&L) has been tested by ELISA, SDS-PAGE, and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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