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Chicken IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Chicken IgG (H&L) antibody (TRITC)

Product Specifications

Product Name Alternative

Goat anti-Chicken IgG Rhodamine Conjugated Antibody, goat anti-Chicken IgG Antibody TRITC Conjugation, Chicken Secondary Antibody, goat anti-Chicken IgY Rhodamine Conjugated Antibody, goat anti-Chicken IgY Antibody TRITC Conjugation

Reactivity

Gallus

Immunogen

Chicken IgG whole molecule

Clonality

Polyclonal

Conjugation

TRITC

Concentration

1.0 mg/mL

Dilution

FLISA: 1:10,000 - 1:50,000, IF: 1:1,000 - 1:5,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Tested Applications

FLISA, IF

Host or Source

Goat

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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