Cat IgG (H&L) Antibody Peroxidase Conjugated
Cat IgG (H&L) antibody (Peroxidase)
Product Specifications
Product Name Alternative
Goat anti-Cat IgG peroxidase Conjugated Antibody, Goat anti-Cat IgG HRP Conjugated Antibody
Reactivity
Feline
Immunogen
Cat IgG whole molecule
Clonality
Polyclonal
Conjugation
HRP
Field of Research
Metabolism Research
Concentration
2.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Cat IgG and Cat Serum.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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