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Bovine IgG Fc Antibody Biotin Conjugated

Bovine IgG F (c) antibody (Biotin)

Product Specifications

Product Name Alternative

Rabbit anti Bovine IgG F (c) Antibody Biotin Conjugated, rabbit anti Bovine IgG Fc Antibody Biotin Conjugated, rabbit anti Bovine IgG Fc fragment biotin conjugate

Reactivity

Bovine

Immunogen

Bovine IgG F (c) fragment

Clonality

Polyclonal

Conjugation

Biotin

Concentration

1.5 mg/mL

Dilution

ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG, Bovine IgG F (c) and Bovine Serum. No reaction was observed against Bovine IgG F (ab) .

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:15,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent) . Reconstitution Volume: 1.0 mL

Tested Applications

ELISA, IHC, WB

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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