Lep Antibody

Lep antibody

Product Specifications

Product Name Alternative

Rabbit anti-Leptin, LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody

UniProt

P41160

Reactivity

Human, Mouse

Immunogen

This whole rabbit serum was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.

Target

Lep

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

85 mg/mL

Dilution

ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000

Purity

This antiserum has been heated to 56°C for 30 minutes. The antibody will recognize recombinant and native 16 kDa leptin from mouse and human. Reactivity with leptin from other sources is unknown.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This antiserum against Mouse Leptin has been tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. This product has been assayed by immunoblot against tissue homogenates using HRP Goat-anti-Rabbit IgG [H&L] and TMB as a substrate. A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against recombinant mouse leptin using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2,400 is suggested for this product.