RFX5 Antibody

Rabbit anti-RFX5 Antibody, DNA binding protein RFX5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, MHC

Anti-RFX5 Antibody was prepared by repeated immunizations of an RFX5 peptide conjugate and purified as monospecific antiserum after delipidation and defibrination.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-RFX5 Antibody was tested by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG. Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. Anti-RFX5 Antibody was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 μl per assay. Specific conditions should be optimized by user. Other assays should be optimized by researcher.