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CHK1 Antibody

CHK1 antibody

Product Specifications

Product Name Alternative

Rabbit anti-CHK1 Antibody, CHK-1, CHK 1, Serine/threonine-protein kinase CHK1, Checkpoint kinase 1

UniProt

P38147

Reactivity

Yeast

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 305-330 of S. cerevisiae CHK1.

Target

CHK1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Concentration

0.73 mg/mL

Dilution

ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:200 - 1:2,000

Purity

This affinity purified antibody is directed against yeast CHK1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against yeast CHK1 protein. A BLAST analysis was used to suggest little to no cross reactivity with this protein from other sources.

Form

Liquid (sterile filtered)

Storage Conditions

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 40-60 kDa in size corresponding to CHK1 by western blotting in the appropriate cell lysate or extract.

Tested Applications

ELISA, IP, WB

NCBI Accession Number

6319751

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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