CHK1 Antibody
CHK1 antibody
Product Specifications
Product Name Alternative
Rabbit anti-CHK1 Antibody, CHK-1, CHK 1, Serine/threonine-protein kinase CHK1, Checkpoint kinase 1
UniProt
P38147
Reactivity
Yeast
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 305-330 of S. cerevisiae CHK1.
Target
CHK1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Concentration
0.73 mg/mL
Dilution
ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:200 - 1:2,000
Purity
This affinity purified antibody is directed against yeast CHK1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against yeast CHK1 protein. A BLAST analysis was used to suggest little to no cross reactivity with this protein from other sources.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 40-60 kDa in size corresponding to CHK1 by western blotting in the appropriate cell lysate or extract.
Tested Applications
ELISA, IP, WB
NCBI Accession Number
6319751
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG
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