WRNIP1 Antibody

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase. Reactivity is expected against human, mouse, rat and monkey WHIP1 protein.

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody has been tested by WB and ELISA. Anti-WHIP is useful in western blotting against HEK293 whole cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 96.0 kDa (predicted molecular weight: 72.2 kDa) . Specific band detection by western blot is blocked by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.