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SPANXC Antibody

SPANX-C antibody

Product Specifications

Product Name Alternative

Rabbit anti-SPANX-C antibody, Cancer-testis-associated protein CTp11 antibody, CTP11 antibody, Nuclear associated protein SPAN-Xc antibody, SPANX family member C antibody, Sperm protein associated with the nucleus on the X chromosome C, SPANX family member C1 antibody, SPANXC

UniProt

Q9NY87

Reactivity

Human

Immunogen

This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human SPANX-C protein.

Target

SPANXC

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

5.0 mg/mL

Dilution

ELISA: 1:5,000 - 1:20,000, IHC: 1.25-2.5 μg/ml, WB: 1:1,000 - 1:5,000

Purity

This Protein A purified antibody is directed against human SPANX-C protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above. A protein-protein BLAST analysis was used to suggest cross reactivity with most SPANX proteins from human, gorilla and chimpanzee. Reactivity against homologues from is not known.

Form

Lyophilized

Storage Conditions

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Notes

For research use only.

Applications Notes

This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15-20 kDa in size corresponding to SPANX family members by western blotting in the appropriate human tissue. Most human cell lines are SPANX negative.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

6977870

Host or Source

Rabbit

Preservative

Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Isotype

IgG

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