F (ab') 2 Rat IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed
Selected Variant
GTR18186383
Size: 500 µg
F (ab') 2 Rat IgG (H&L) antibody (Biotin)
Product Specifications
Product Name Alternative
Donkey F (ab') 2 Anti-Rat IgG Biotin Conjugated Pre-Adsorbed Antibody, Donkey Fab2 Anti-Rat IgG Antibody Biotin Conjugation
Reactivity
Rat
Immunogen
Rat IgG whole molecule
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,000, WB: 1:1,000 - 1:5,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F (c), or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep serum proteins.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Tested Applications
ELISA, IHC, WB
Host or Source
Donkey
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG F (ab') 2
Price
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