F (ab') 2 MOUSE IgM (mu chain) Antibody Peroxidase Conjugated
F (ab') 2 MOUSE IgM (mu chain) antibody (Peroxidase)
Product Specifications
Product Name Alternative
Goat F (ab') 2 Anti-Mouse IgM (mu chain) Antibody Peroxidase Conjugation, Goat Fab2 Anti-Mouse IgM mu chain PE Conjugated Antibody
Reactivity
Mouse
Immunogen
Mouse IgM whole molecule
Clonality
Polyclonal
Conjugation
HRP
Concentration
1.0 mg/mL
Dilution
ELISA: 1:25,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F (c) . No reaction was observed against other mouse heavy or light chain proteins.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
F (ab') 2 Anti-Mouse IgM mu heavy chain peroxidase conjugated antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Tested Applications
ELISA, IHC, WB
Host or Source
Goat
Preservative
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG F (ab') 2
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